Studies in synthetic immunochemistry

Cxlvi. Studies in Synthetic Immunochemistry

IN the first paper of this series [Clutton et al. 1937] a method was described by which simple carbohydrate residues could be introduced into a protein in the form of the O-fl-glycoside of tyrosine, the latter being coupled in peptide linkage with the free amino groups of the protein molecule. Application of this method to the preparation of O-fl-glucosidotyrosylgelatin was described and it was...

Studies on the Immunochemistry of Streptococcal Mucopeptide

Streptococcal mucopeptide, solubilized by either ultrasonic treatment or lysozyme, gave a precipitin reaction with rabbit antimucopeptide serum. A haptenic inhibitor of this reaction, which was composed of alanine, glutamic acid, and lysine in a mole ratio of 4:1:1, was isolated from a Streptomyces albus enzymes digest of Group D cell walls by ion exchange chromatography. When selected antisera...

Immunochemistry.

Immunochemistry.

Immunochemistry of cartilage proteoglycan. Immunodiffusion and gel-electrophoretic studies.

Cartilage proteoglycan is thought to be composed of subunits, core proteins with covalently attached sulphated polysaccharide side chains, which form aggregates by non-covalent association with a link protein. The new technique of non-disruptive extraction followed by fractionation in caesium chloride gradients provides a useful means of preparing relatively pure proteoglycan aggregate, subunit...